Article Figures & Data
- Figure 1
Schematic representation of the experimental design. mNSS, Modified Neurological Severity Score; MSC, mesenchymal stem cells. The figure was created with BioRender.com under a paid subscription.
- Figure 2
Post stroke brain damage and swelling. (A) Representative images of TTC-stained serial coronal brain sections from untreated male and female ischaemic rats. Animals were subjected to a 2-hour ischaemia and were subsequently euthanised 1 day after reperfusion. Sections are shown anterior to posterior (top to bottom) and span most of the forebrain from approximately +2 mm to −4 mm from bregma. The red-stained areas indicate normal healthy tissue, whereas the unstained (white) areas ipsilateral to MCAO indicate infarcted tissue. The column scatter plots show the total infarct volume (B) and swelling (D) at 1 day after reperfusion in untreated male and female ischaemic rats. Values are expressed as mean±SEM. Group N’s were six males and six females for infarct volume and five males and six females for swelling. No statistically significant differences were found between the two groups in either infarct volume or brain swelling (p>0.05, unpaired t-test). The bar graphs show the section-by-section analysis of TTC staining in untreated male and female ischaemic rats for infarct volume (C) and swelling (E). Values are expressed as mean+SEM (group N’s as above). Two-way repeated measures analysis of variance did not reveal any significant differences between the two groups in either infarct volume or swelling. MCAO, middle cerebral artery occlusion; TTC, triphenyltetrazolium chloride.
- Figure 3
Post stroke changes in Modified Neurological Severity Score. The bar graph shows the neurological scores of rats subjected to a 2-hour ischaemia and subsequently treated with MSC or vehicle control 1 day after reperfusion. Values are expressed as mean+SEM. Group N’s were 13 for the Vehicle Male group, 14 for the Vehicle Female group, 12 for the MSC Male group and 9 for the MSC Female group except for the 14-day timepoint where the group N’s were 10 (Vehicle Males), 13 (Vehicle Females), 8 (MSC Males) and 7 (MSC Females). Data were analysed by two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test (for the 1–7-day timepoints) or by two-way ANOVA followed by Sidak’s post hoc test (for the 14-day timepoint). ****p<0.0001 versus the same-sex vehicle group. +++p<0.001; ++++p<0.0001 versus males receiving the same treatment. ANOVA, analysis of variance; MSC, mesenchymal stem cells.
- Figure 4
Treatment with MSC promotes the recovery of somatosensory function. The line graph shows the sticky-tape ratios of rats subjected to a 2-hour ischaemia and subsequently treated with MSC or vehicle control 1 day after reperfusion. Values are expressed as mean+SEM. Group N’s were 13 for the Vehicle Male group, 14 for the Vehicle Female group, 12 for the MSC Male group and 9 for the MSC Female group except for the 14-day timepoint where the N’s were 7 (Vehicle Males), 10 (Vehicle Females), 8 (MSC Males) and 7 (MSC Females). Data were analysed by two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test (for the 1–7-day timepoints) or two-way ANOVA followed by Sidak’s post hoc test (for the 14-day timepoint). *p<0.05; **p<0.01; ***p<0.001 versus the same-sex vehicle group. +p<0.05; ++p<0.01 versus males receiving the same treatment. ANOVA, analysis of variance; MSC, mesenchymal stem cells.
- Figure 5
MSC treatment improves post stroke beam walk scores and rotarod performance. The line graphs show the beam walk scores (A) or rotarod latencies (expressed as a percent of baseline) (B) of rats subjected to a 2-hour ischaemia and subsequently treated with MSC or vehicle control 1 day after reperfusion. Values are expressed as mean+SEM. For the beam walk test, group N’s were 13 for the Vehicle Male group, 14 for the Vehicle Female group, 12 for the MSC Male group and 9 for the MSC Female group except for the 14-day timepoint where the N’s were 10 (Vehicle Males), 13 (Vehicle Females), 8 (MSC Males) and 7 (MSC Females). For the rotarod test, group N’s were 10 for the Vehicle Male group, 14 for the Vehicle Female group, 10 for the MSC Male group and 9 for the MSC Female group except for the 14-day timepoint where the N’s were 8 (Vehicle Males), 13 (Vehicle Females), 8 (MSC Males) and 7 (MSC Females). The horizontal line in each graph indicates the pre ischaemic baseline level. Data were analysed by two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test (for the 1−7-day timepoints) or by two-way ANOVA followed by Sidak’s post hoc test (for the 14-day timepoint). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001 versus the same-sex vehicle group. +p<0.05; ++p<0.01 versus males receiving the same treatment. ANOVA, analysis of variance; MSC, mesenchymal stem cells.
Supplementary Materials
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Supplementary Data
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