Article Figures & Data
Figures
- Figure 1
Timeline for HPF labelling MSCs. When the cultured MSCs grow to 80%–90% density, heparin, protamine and ferulic acid are added in sequence and mixed for 5 min. After 2–4 hours of cocultivation, 10%–20% FBS was added and the culture was continued for 24 hours. After washing with PBS and heparin, the culture was continued for 15 min to obtain HPF nanocomplex. HPF, ferumoxytol–heparin–protamine; MSC, mesenchymalstem cell;FBS, fatal bovine serun; PBS, phosphate buffered solution.
- Figure 2
HPF nanocomplex combined with MRI can track and monitor MSCs. (A). The images show HPF-labelled and unlabelled MscS in the tube in vitro by the T2*-weighted MRI. (B.) the images show HPF-labelled and unlabelled MscS in the brain striatum of rats on the third day after stereotactic injection by the T2*-weighted MRI. HPF, ferumoxytol–heparin–protamine; MSC, mesenchymalstem cell.
Tables
- Table 1
Labelling and tracking stem cells in neurological diseases
Disease Cell type Tracking technology Tracer Species References Stroke MSC MRI Molday ION-Rhodamine B Pigs/dogs/ Rats Walczak et al, 201751 Stroke MSC MRI/NIRF BCN-dual-NPs Mice Lim et al, 201952 Stroke MSC MRI/PAI GRMNBs Mice Chen et al, 201553 Stroke NSC MRI/FLI LV-FTH-EGFP Mice Zhang et al, 201754 Stroke MSC MRI/SPECT/CT 125I-fSiO4@SPIOs Rats Tang et al, 201555 PD NSC MRI MNP Rats Gomez et al, 201510 AD MSC MRI Ferumoxytol Mice Lee et al, 201767 ALS hGRP MRI PFC Mice Richard et al, 201972 TBI iPS cell MRI SPIO Rats Jiang et al, 201978 SCI BM-MSC MRI Gd-DTPA-FA Rats Zhang et al, 201786 AD, Alzheimer’s disease; ALS, amyotrophic lateral sclerosis; BCN-dual-NPs, bicyclic nonyne conjugated ethylene glycol chitosan nanoparticles; BM-MSC, bone marrow mesenchymal stem cell; iPS cell, induced pluripotent stem cell; FLI, fluorescence imaging; Gd-DTPA-FA, gadolinium-dimethylene penta-acetic acid-containing nanoparticles; GRMNBs, multi-gold nanorods crystal-seeded magnetic mesoporous silica nanobeads; hGRP, human glial-restricted progenitor; 125I-fSiO4@SPIOs, fluorescent silica-coated SPIOs with 125iodine; LV-FTH-EGFP, a lentiviral vector encoding ferritin heavy chain (FTH) and enhanced green fluorescent protein (EGFP); MNP, magnetic nanoparticle; MSC, mesenchymal stem cells; NIRF, near-infrared fluorescent; NSC, neural stem cell; PAI, photoacoustic imaging; PD, Parkinson’s disease; PET, positron emission tomography; PFC, perfluorocarbon; SCI, spinal cord injury; SPECT, single-photon emission CT; SPIO, superparamagnetic iron oxide; TBI, traumatic brain injury.
