PT  - JOURNAL ARTICLE
AU  - Zhang, Jie
AU  - Li, Haiying
AU  - Xu, Zhongmou
AU  - Lu, Jinxin
AU  - Cao, Chang
AU  - Shen, Haitao
AU  - Li, Xiang
AU  - You, Wanchun
AU  - Chen, Gang
TI  - Oestrogen ameliorates blood-brain barrier damage after experimental subarachnoid haemorrhage via the SHH pathway in male rats
AID  - 10.1136/svn-2022-001907
DP  - 2023 Jun 01
TA  - Stroke and Vascular Neurology
PG  - 217--228
VI  - 8
IP  - 3
4099  - http://svn.bmj.com/content/8/3/217.short
4100  - http://svn.bmj.com/content/8/3/217.full
SO  - Stroke Vasc Neurol2023 Jun 01; 8
AB  - Background Sex differences affect the occurrence, progression and regression of subarachnoid haemorrhage (SAH). Oestrogen plays a protective role in alleviating the vasospasm and neuronal apoptosis induced by SAH. However, whether oestrogen affects blood‒brain barrier (BBB) integrity has not been fully studied. Oestrogen has been found to regulate the sonic hedgehog (SHH) signalling pathway through the oestrogen receptor in gastric cancer and adrenal glands, and the SHH signalling pathway has an important role in maintaining the BBB by upregulating the expression of tight junction proteins. In this study, we investigated the relationship between oestrogen and the SHH signalling pathway using clinical data and established an experimental SAH model to explore whether oestrogen could ameliorate BBB damage after SAH through the SHH pathway.Methods Correlations between oestrogen and the SHH pathway were analysed by patients’ cerebrospinal fluid (CSF) samples and the Genotype-Tissue Expression database (GTEx). Then, an experimental rat SAH model was established using the endovascular perforation method and treated with oestrogen, oestrogen inhibitors and SHH signalling pathway inhibitors. Then, the effects of oestrogen on BBB damage were analysed by western blot, immunofluorescence and neurobehavioural experiments.Results ESLIA detection and correlation analysis showed that oestrogen levels in patients’ CSF were positively correlated with the SHH pathway, which was further verified by GTEx gene-correlation analysis. SHH was found to be mainly expressed in neurons and astrocytes in rats under physiological conditions and was upregulated by oestrogen pretreatment. In the SAH model, oestrogen pretreatment was found to reverse SAH-induced decreases in the SHH pathway, which were counteracted by oestrogen receptor inhibitors. Furthermore, oestrogen pretreatment reduced SAH-induced BBB damage, brain oedema and neurological dysfunction, which were eliminated by SHH pathway inhibitors.Conclusion In conclusion, we demonstrate here that oestrogen pretreatment ameliorates brain injury after SAH, at least in part through SHH pathway-mediated BBB protection.Data are available on reasonable request.