RT Journal Article
SR Electronic
T1 Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats
JF Stroke and Vascular Neurology
JO Stroke Vasc Neurol
FD BMJ Publishing Group Ltd
SP 381
OP 389
DO 10.1136/svn-2021-001231
VO 7
IS 5
A1 Liu, Chang
A1 Cheng, Xi
A1 Zhong, Shanshan
A1 Liu, Zhouyang
A1 Liu, Fangxi
A1 Lin, Xinyu
A1 Zhao, Yinan
A1 Guan, Meiting
A1 Xiao, Ting
A1 Jolkkonen, Jukka
A1 Wang, Ying
A1 Zhao, Chuansheng
YR 2022
UL http://svn.bmj.com/content/7/5/381.abstract
AB Background The brain-gut axis is a major regulator of the central nervous system. We investigated the effects of treatment with broad-spectrum antibiotics on gut and brain inflammation, infarct size and long-term behavioral outcome after cerebral ischemia in rats.Methods Rats were treated with broad-spectrum antibiotics (ampicillin, vancomycin, ciprofloxacin, meropenem and metronidazole) for 4 weeks before the endothelin-1 induced ischemia. Treatment continued for 2 weeks until the end of behavioral testing, which included tapered ledged beam-walking, adhesive label test and cylinder test. Gut microbiome, short-chain fatty acids and cytokine levels were measured together with an assessment of infarct size, neuroinflammation and neurogenesis.Results The results revealed that the antibiotics exerted a clear impact on the gut microbiota. This was associated with a decrease in systemic and brain cytokine levels, infarct size and apoptosis in the perilesional cortex and improved behavioral outcome.Conclusion Our results highlighted the significant relationship between intestinal microbiota and beneficial neuro-recovery after ischemic stroke.Data are available upon reasonable request. The data contains the original images as well as the original files, the raw data are available from the authors CL and CZ (leo_liuchang@163.com and cszhao@cmu.edu.cn). Permission to reuse the data need to be granted with the permission of the journal and the corresponding author.